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murine specific apoe  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology murine specific apoe
    <t>ABCA1</t> protein levels were determined in ipsilateral half brains of WT ( A ) and <t>apoE−/−</t> ( B ) mice following mrTBI using Western blots, with representative blots shown below the graphs. Data are expressed as fold difference normalized to sham values. Data from sham animals within each genotype were pooled (grey bars). Numbers inside the bars indicate sample size. Asterisks above individual bars indicate significant difference compared to the respective sham levels. *: p <0.05, **: p <0.01, ***: p <0.001, ****: p <0.0001. Data were analyzed by two-way ANOVA followed by a Bonferroni post hoc test. Legend: S: sham V: untreated mice, open bars, G: GW3965-treated mice, black bars.
    Murine Specific Apoe, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+specific+apoe+antibody/apoE+Antibody/pmc03547922-205-32-37
    Average 95 stars, based on 247 article reviews
    murine specific apoe - by Bioz Stars, 2026-10
    95/100 stars

    Images

    1) Product Images from "The Liver X Receptor Agonist GW3965 Improves Recovery from Mild Repetitive Traumatic Brain Injury in Mice Partly through Apolipoprotein E"

    Article Title: The Liver X Receptor Agonist GW3965 Improves Recovery from Mild Repetitive Traumatic Brain Injury in Mice Partly through Apolipoprotein E

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0053529

    ABCA1 protein levels were determined in ipsilateral half brains of WT ( A ) and apoE−/− ( B ) mice following mrTBI using Western blots, with representative blots shown below the graphs. Data are expressed as fold difference normalized to sham values. Data from sham animals within each genotype were pooled (grey bars). Numbers inside the bars indicate sample size. Asterisks above individual bars indicate significant difference compared to the respective sham levels. *: p <0.05, **: p <0.01, ***: p <0.001, ****: p <0.0001. Data were analyzed by two-way ANOVA followed by a Bonferroni post hoc test. Legend: S: sham V: untreated mice, open bars, G: GW3965-treated mice, black bars.
    Figure Legend Snippet: ABCA1 protein levels were determined in ipsilateral half brains of WT ( A ) and apoE−/− ( B ) mice following mrTBI using Western blots, with representative blots shown below the graphs. Data are expressed as fold difference normalized to sham values. Data from sham animals within each genotype were pooled (grey bars). Numbers inside the bars indicate sample size. Asterisks above individual bars indicate significant difference compared to the respective sham levels. *: p <0.05, **: p <0.01, ***: p <0.001, ****: p <0.0001. Data were analyzed by two-way ANOVA followed by a Bonferroni post hoc test. Legend: S: sham V: untreated mice, open bars, G: GW3965-treated mice, black bars.

    Techniques Used: Western Blot

    Related Articles

    other:

    Article Title: Lack of ABCA1 Considerably Decreases Brain ApoE Level and Increases Amyloid Deposition in APP23 Mice
    Article Snippet: Murine-specific apoE antibody was from Santa Cruz Biotechnology (Santa Cruz, CA).

    Western Blot:

    Article Title: ATP-binding Cassette Transporter A1 Mediates the Beneficial Effects of the Liver X Receptor Agonist GW3965 on Object Recognition Memory and Amyloid Burden in Amyloid Precursor Protein/Presenilin 1 Mice*
    Article Snippet: Protein concentration was determined by DC Protein assay (Bio-Rad). .. Western Blot Analysis of ABCA1, ApoE, APP, and APP-CTF— For analysis ofABCA1, apoE, andAPP, 50–100 g of carbonate lysate was electrophoresed through 10% SDS-polyacrylamide gels, transferred to PVDFmembrane (Millipore), and immunodetected using a monoclonal anti-ABCA1 antibody, AC10 (1:1000, a kind gift from Dr. M. R. Hayden), a murine-specific apoE antibody (1:1000, Santa Cruz Biotechnology), an antiAPP antibody that recognizes both murine and human APP (clone 22C11, 1:2000, Chemicon), or an anti-actin antibody (1:5000, Chemicon) as a loading control. .. APP and APP-CTF were analyzed by resolving 25 g of carbonate lysate through 4–12% NuPAGE BisTris gradient gels (Invitrogen, NP0335).

    Article Title: ATP-binding Cassette Transporter A1 Mediates the Beneficial Effects of the Liver X Receptor Agonist GW3965 on Object Recognition Memory and Amyloid Burden in Amyloid Precursor Protein/Presenilin 1 Mice
    Article Snippet: Protein concentration was determined by DC Protein assay (Bio-Rad). .. Western Blot Analysis of ABCA1, ApoE, APP, and APP-CTF For analysis of ABCA1 , apoE , and APP, 50–100 μg of carbonate lysate was electrophoresed through 10% SDS-polyacrylamide gels, transferred to PVDF membrane (Millipore), and immunodetected using a monoclonal anti- ABCA1 antibody, AC10 (1:1000, a kind gift from Dr. M. R. Hayden), a murine-specific apoE antibody (1:1000, Santa Cruz Biotechnology), an anti-APP antibody that recognizes both murine and human APP (clone 22C11, 1:2000, Chemicon), or an anti-actin antibody (1:5000, Chemicon) as a loading control. .. APP and APP-CTF were analyzed by resolving 25 μg of carbonate lysate through 4–12% NuPAGE BisTris gradient gels (Invitrogen, NP0335).

    Control:

    Article Title: ATP-binding Cassette Transporter A1 Mediates the Beneficial Effects of the Liver X Receptor Agonist GW3965 on Object Recognition Memory and Amyloid Burden in Amyloid Precursor Protein/Presenilin 1 Mice*
    Article Snippet: Protein concentration was determined by DC Protein assay (Bio-Rad). .. Western Blot Analysis of ABCA1, ApoE, APP, and APP-CTF— For analysis ofABCA1, apoE, andAPP, 50–100 g of carbonate lysate was electrophoresed through 10% SDS-polyacrylamide gels, transferred to PVDFmembrane (Millipore), and immunodetected using a monoclonal anti-ABCA1 antibody, AC10 (1:1000, a kind gift from Dr. M. R. Hayden), a murine-specific apoE antibody (1:1000, Santa Cruz Biotechnology), an antiAPP antibody that recognizes both murine and human APP (clone 22C11, 1:2000, Chemicon), or an anti-actin antibody (1:5000, Chemicon) as a loading control. .. APP and APP-CTF were analyzed by resolving 25 g of carbonate lysate through 4–12% NuPAGE BisTris gradient gels (Invitrogen, NP0335).

    Article Title: The LXR agonist GW3965 increases apoA-I protein levels in the central nervous system independent of ABCA1.
    Article Snippet: Contents lists available at SciVerse ScienceDirect Biochimica et Biophysica Acta j ourna l homepage: www.e lsev ie r .com/ locate /bba l ip The LXR agonist GW3965 increases apoA-I protein levels in the central nervous system independent of ABCA1☆ Sophie Stukas, Sharon May, Anna Wilkinson, Jeniffer Chan, James Donkin, Cheryl L. Wellington ⁎ Department of Pathology and Laboratory Medicine, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada V5Z 4H4 ☆ This article is part of a Special Issue entitled Advanc Formation and Metabolism: A Tribute to John F. Oram ( ⁎ Corresponding author at: Department of Patholo University of British Columbia, 980 West 28th Avenue, V Tel.. : +1 604 875 2000x6825; fax: +1 604 875 3120.. E-mail address: Cheryl@cmmt.ubc.ca (C.L.

    Article Title: The Absence of ABCA1 Decreases Soluble ApoE Levels but Does Not Diminish Amyloid Deposition in Two Murine Models of Alzheimer Disease
    Article Snippet: For soluble apoE (PBS fraction), ABCA1, and APP (lysis buffer fractions), protein concentrations were determined by DC protein assay (Bio-Rad) prior to analysis. .. Equal amounts of protein were electrophoresed through 10% SDS-polyacrylamide gels, electrophoretically transferred to polyvinylidene fluoride membrane (Millipore), and immunodetected using a murine-specific apoE antibody (Santa Cruz Biotechnology), a monoclonal anti-ABCA1 antibody raised against the second nucleotide binding domain (48), 22C11, which detects both murine and human APP (Chemicon), or an anti-GAPDH antibody (Chemicon) as a loading control. .. Blots were developed using enhanced chemiluminescence (Amersham Biosciences) according to the manufacturer’s recommendations.

    Article Title: ATP-binding Cassette Transporter A1 Mediates the Beneficial Effects of the Liver X Receptor Agonist GW3965 on Object Recognition Memory and Amyloid Burden in Amyloid Precursor Protein/Presenilin 1 Mice
    Article Snippet: Protein concentration was determined by DC Protein assay (Bio-Rad). .. Western Blot Analysis of ABCA1, ApoE, APP, and APP-CTF For analysis of ABCA1 , apoE , and APP, 50–100 μg of carbonate lysate was electrophoresed through 10% SDS-polyacrylamide gels, transferred to PVDF membrane (Millipore), and immunodetected using a monoclonal anti- ABCA1 antibody, AC10 (1:1000, a kind gift from Dr. M. R. Hayden), a murine-specific apoE antibody (1:1000, Santa Cruz Biotechnology), an anti-APP antibody that recognizes both murine and human APP (clone 22C11, 1:2000, Chemicon), or an anti-actin antibody (1:5000, Chemicon) as a loading control. .. APP and APP-CTF were analyzed by resolving 25 μg of carbonate lysate through 4–12% NuPAGE BisTris gradient gels (Invitrogen, NP0335).

    Article Title: ATP-binding Cassette Transporter A1 Mediates the Beneficial Effects of the Liver X Receptor Agonist GW3965 on Object Recognition Memory and Amyloid Burden in Amyloid Precursor Protein/Presenilin 1 Mice
    Article Snippet: Protein concentration was determined by DC Protein assay (Bio-Rad). .. For analysis of ABCA1 , apoE , and APP, 50–100 μg of carbonate lysate was electrophoresed through 10% SDS-polyacrylamide gels, transferred to PVDF membrane (Millipore), and immunodetected using a monoclonal anti- ABCA1 antibody, AC10 (1:1000, a kind gift from Dr. M. R. Hayden), a murine-specific apoE antibody (1:1000, Santa Cruz Biotechnology), an anti-APP antibody that recognizes both murine and human APP (clone 22C11, 1:2000, Chemicon), or an anti-actin antibody (1:5000, Chemicon) as a loading control. .. APP and APP-CTF were analyzed by resolving 25 μg of carbonate lysate through 4–12% NuPAGE BisTris gradient gels (Invitrogen, NP0335).

    Membrane:

    Article Title: The Absence of ABCA1 Decreases Soluble ApoE Levels but Does Not Diminish Amyloid Deposition in Two Murine Models of Alzheimer Disease
    Article Snippet: For soluble apoE (PBS fraction), ABCA1, and APP (lysis buffer fractions), protein concentrations were determined by DC protein assay (Bio-Rad) prior to analysis. .. Equal amounts of protein were electrophoresed through 10% SDS-polyacrylamide gels, electrophoretically transferred to polyvinylidene fluoride membrane (Millipore), and immunodetected using a murine-specific apoE antibody (Santa Cruz Biotechnology), a monoclonal anti-ABCA1 antibody raised against the second nucleotide binding domain (48), 22C11, which detects both murine and human APP (Chemicon), or an anti-GAPDH antibody (Chemicon) as a loading control. .. Blots were developed using enhanced chemiluminescence (Amersham Biosciences) according to the manufacturer’s recommendations.

    Article Title: ATP-binding Cassette Transporter A1 Mediates the Beneficial Effects of the Liver X Receptor Agonist GW3965 on Object Recognition Memory and Amyloid Burden in Amyloid Precursor Protein/Presenilin 1 Mice
    Article Snippet: Protein concentration was determined by DC Protein assay (Bio-Rad). .. Western Blot Analysis of ABCA1, ApoE, APP, and APP-CTF For analysis of ABCA1 , apoE , and APP, 50–100 μg of carbonate lysate was electrophoresed through 10% SDS-polyacrylamide gels, transferred to PVDF membrane (Millipore), and immunodetected using a monoclonal anti- ABCA1 antibody, AC10 (1:1000, a kind gift from Dr. M. R. Hayden), a murine-specific apoE antibody (1:1000, Santa Cruz Biotechnology), an anti-APP antibody that recognizes both murine and human APP (clone 22C11, 1:2000, Chemicon), or an anti-actin antibody (1:5000, Chemicon) as a loading control. .. APP and APP-CTF were analyzed by resolving 25 μg of carbonate lysate through 4–12% NuPAGE BisTris gradient gels (Invitrogen, NP0335).

    Article Title: ATP-binding Cassette Transporter A1 Mediates the Beneficial Effects of the Liver X Receptor Agonist GW3965 on Object Recognition Memory and Amyloid Burden in Amyloid Precursor Protein/Presenilin 1 Mice
    Article Snippet: Protein concentration was determined by DC Protein assay (Bio-Rad). .. For analysis of ABCA1 , apoE , and APP, 50–100 μg of carbonate lysate was electrophoresed through 10% SDS-polyacrylamide gels, transferred to PVDF membrane (Millipore), and immunodetected using a monoclonal anti- ABCA1 antibody, AC10 (1:1000, a kind gift from Dr. M. R. Hayden), a murine-specific apoE antibody (1:1000, Santa Cruz Biotechnology), an anti-APP antibody that recognizes both murine and human APP (clone 22C11, 1:2000, Chemicon), or an anti-actin antibody (1:5000, Chemicon) as a loading control. .. APP and APP-CTF were analyzed by resolving 25 μg of carbonate lysate through 4–12% NuPAGE BisTris gradient gels (Invitrogen, NP0335).

    Binding Assay:

    Article Title: The Absence of ABCA1 Decreases Soluble ApoE Levels but Does Not Diminish Amyloid Deposition in Two Murine Models of Alzheimer Disease
    Article Snippet: For soluble apoE (PBS fraction), ABCA1, and APP (lysis buffer fractions), protein concentrations were determined by DC protein assay (Bio-Rad) prior to analysis. .. Equal amounts of protein were electrophoresed through 10% SDS-polyacrylamide gels, electrophoretically transferred to polyvinylidene fluoride membrane (Millipore), and immunodetected using a murine-specific apoE antibody (Santa Cruz Biotechnology), a monoclonal anti-ABCA1 antibody raised against the second nucleotide binding domain (48), 22C11, which detects both murine and human APP (Chemicon), or an anti-GAPDH antibody (Chemicon) as a loading control. .. Blots were developed using enhanced chemiluminescence (Amersham Biosciences) according to the manufacturer’s recommendations.



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    95
    Santa Cruz Biotechnology murine specific apoe
    <t>ABCA1</t> protein levels were determined in ipsilateral half brains of WT ( A ) and <t>apoE−/−</t> ( B ) mice following mrTBI using Western blots, with representative blots shown below the graphs. Data are expressed as fold difference normalized to sham values. Data from sham animals within each genotype were pooled (grey bars). Numbers inside the bars indicate sample size. Asterisks above individual bars indicate significant difference compared to the respective sham levels. *: p <0.05, **: p <0.01, ***: p <0.001, ****: p <0.0001. Data were analyzed by two-way ANOVA followed by a Bonferroni post hoc test. Legend: S: sham V: untreated mice, open bars, G: GW3965-treated mice, black bars.
    Murine Specific Apoe, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+specific+apoe+antibody/apoE+Antibody/pmc03547922-205-32-37
    Average 95 stars, based on 1 article reviews
    murine specific apoe - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    95
    Santa Cruz Biotechnology murine specific apoe antibody
    <t>ABCA1</t> protein levels were determined in ipsilateral half brains of WT ( A ) and <t>apoE−/−</t> ( B ) mice following mrTBI using Western blots, with representative blots shown below the graphs. Data are expressed as fold difference normalized to sham values. Data from sham animals within each genotype were pooled (grey bars). Numbers inside the bars indicate sample size. Asterisks above individual bars indicate significant difference compared to the respective sham levels. *: p <0.05, **: p <0.01, ***: p <0.001, ****: p <0.0001. Data were analyzed by two-way ANOVA followed by a Bonferroni post hoc test. Legend: S: sham V: untreated mice, open bars, G: GW3965-treated mice, black bars.
    Murine Specific Apoe Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+specific+apoe+antibody/apoE+Antibody/pm21889608-83-31-35
    Average 95 stars, based on 1 article reviews
    murine specific apoe antibody - by Bioz Stars, 2026-10
    95/100 stars
      Buy from Supplier

    Image Search Results


    ABCA1 protein levels were determined in ipsilateral half brains of WT ( A ) and apoE−/− ( B ) mice following mrTBI using Western blots, with representative blots shown below the graphs. Data are expressed as fold difference normalized to sham values. Data from sham animals within each genotype were pooled (grey bars). Numbers inside the bars indicate sample size. Asterisks above individual bars indicate significant difference compared to the respective sham levels. *: p <0.05, **: p <0.01, ***: p <0.001, ****: p <0.0001. Data were analyzed by two-way ANOVA followed by a Bonferroni post hoc test. Legend: S: sham V: untreated mice, open bars, G: GW3965-treated mice, black bars.

    Journal: PLoS ONE

    Article Title: The Liver X Receptor Agonist GW3965 Improves Recovery from Mild Repetitive Traumatic Brain Injury in Mice Partly through Apolipoprotein E

    doi: 10.1371/journal.pone.0053529

    Figure Lengend Snippet: ABCA1 protein levels were determined in ipsilateral half brains of WT ( A ) and apoE−/− ( B ) mice following mrTBI using Western blots, with representative blots shown below the graphs. Data are expressed as fold difference normalized to sham values. Data from sham animals within each genotype were pooled (grey bars). Numbers inside the bars indicate sample size. Asterisks above individual bars indicate significant difference compared to the respective sham levels. *: p <0.05, **: p <0.01, ***: p <0.001, ****: p <0.0001. Data were analyzed by two-way ANOVA followed by a Bonferroni post hoc test. Legend: S: sham V: untreated mice, open bars, G: GW3965-treated mice, black bars.

    Article Snippet: RIPA lysate (80 μg protein) was electrophoresed through 10% SDS-polyacrylamide gels, transferred to PVDF membrane (Millipore), and immunodetected using following antibodies: monoclonal anti-ABCA1 (AC10, 1∶1000, a gift from Dr. M. R. Hayden), murine-specific apoE (clone M-20, 1∶1000, Santa Cruz Biotechnology, Santa Cruz, CA) and anti-LDLR (1∶1000, R&D Systems).

    Techniques: Western Blot